Practical tool · 1 minute

Melanoma SLNB quality checklist

Seven checkpoints from indication to the pathology report. The checklist does not determine whether a medical error occurred; it helps establish whether the key stages of the process are documented.

Correct indication

Breslow thickness, ulceration and clinical nodal status are known; SLNB is planned before or together with wide local excision.

Ask: why is SLNB recommended in my case?

Lymphatic mapping

The true drainage pathway and nodal basins are defined. The Ukrainian protocol names technetium or indocyanine green for sentinel-node detection.

Ask: which mapping method was used?

Identification of true sentinel nodes

The node or nodes should correspond to the lymphatic map and the criteria of the chosen navigation method.

Ask: how many sentinel nodes were found and in which basins?

Separate submission and labelling

Each sentinel node should be clearly identifiable to pathology by location.

Ask: how many nodes/containers were submitted and how were they labelled?

Adequate gross processing

The Ukrainian protocol specifies nodal tissue pieces no more than 2-3 mm in paraffin blocks. The aim is adequate tissue coverage.

Ask: was the whole sentinel node processed using a dedicated protocol?

Serial levels + H&E + IHC

Ukrainian minimum: at least 3 levels, H&E and IHC, and at least 3 melanocyte-specific antibodies. Exact level spacing follows the validated laboratory protocol.

Ask: which levels and IHC markers were used?

Complete pathology report

Number examined and positive, largest deposit size, location, extranodal extension and other relevant tumour-burden descriptors.

Ask: is the report sufficient for accurate pN staging and management?

After surgery

What happens to the sentinel node after removal

Removal of the sentinel lymph node is only part of the procedure. An equally important stage follows: appropriate gross processing, serial sectioning, morphologic assessment and immunohistochemistry according to protocol.

Post-operative assessment of a sentinel lymph node: grossing, serial levels, H&E, IHC and structured reporting
From the removed node to the final report. High-quality SLN assessment includes systematic grossing, multilevel serial sections, H&E, IHC and a structured pathology report.
Important: a result stating “no metastasis identified” is most meaningful when the node has been assessed using an appropriate protocol. Inadequate tissue coverage can miss a small metastatic deposit, while IHC without morphologic context can lead to incorrect interpretation.
When clarification is needed

A checklist is not a legal conclusion

Missing detail in a short report does not prove that a step was omitted. First obtain the full operative note, mapping/nuclear-medicine documentation and pathology records.

Worth clarifying: if the mapping method, number of sentinel nodes or pathology-processing details are absent from the documentation, the completeness of the full SLNB process cannot be assessed from that record alone.